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Datasets associated with Lee et al. Water sources for coastal forest trees correctly identified through centrifugation of xylem water from sapwood cores but not cryogenic vacuum extraction
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Created: | Mar 20, 2025 at 1:11 p.m. | |
Last updated: | Mar 20, 2025 at 1:22 p.m. | |
Citation: | See how to cite this resource | |
Content types: | CSV Content |
Sharing Status: | Discoverable (Accessible via direct link sharing) |
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Abstract
Water stable isotope datasets associated with Lee et al., "Water sources for coastal forest trees correctly identified through centrifugation of xylem water from sapwood cores but not cryogenic vacuum extraction."
Identifying the water sources plants use is central to understanding potential shifts in water cycling with climate change. Studies have shown that the hydrogen isotope composition (δD) and oxygen isotope composition (δ18O) of xylem waters are sensitive to the water extraction method used, potentially obscuring potential water sources. Here, we report consistent differences in the δD and δ18O compositions of xylem water extracted via cryogenic vacuum distillation (CVD) relative to centrifugation for Douglas-firs and Tanoaks in an old-growth forest in Mendocino county, California across wet and dry seasons. On average, centrifuge extracted samples are more enriched, and the offset in δD between centrifuge and CVD extracted samples is 24‰ for Douglas fir and 15‰ for Tanoak. The effect on δ18O is smaller as a fraction of the mean measurement values, but is still significant for Douglas fir at 0.85‰ and potentially significant for Tanoak at 0.42‰. The xylem water composition of CVD extracted water before and after centrifugation was similar, with the exception of a 5.4‰ offset in δD for Douglas fir, suggesting that centrifugation removes a small but isotopically distinct volume of water from the xylem. Differences in the magnitude of isotopic offset between Douglas fir and Tanoak suggests that species differences in xylem structure may underlie the source of the offset. When compared with water sources at the extensively characterized field site, the centrifuge extracted xylem water δD and δ18O align well with recent rainfall during the wet season and lysimeter extracted moisture from the bedrock root-zone during the dry season. In contrast, xylem water and soil moisture extracted via CVD show similarities in both δD and δ18O, but neither align with rainfall, lysimeter samples, or an evaporative enrichment signal of rainfall for the site. We conclude that CVD extracted xylem is offset from source water in both δD and δ18O and centrifugation of xylem provides a more accurate method for identifying plant water sources. We recommend testing multiple extraction methods to assess potential isotopic differences between methods. This comparative approach allows for understanding method specific biases in δD and δ18O values. Further, when selecting a method for xylem water extraction, researchers should account for species-specific xylem structure which could yield isotopically distinct water pools.
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README.txt
README Overview This repository contains hydrologic data collected from the Rivendell field site at the Eel River Critical Zone Observatory as described in Lee et al. Water sources for coastal forest trees correctly identified through centrifugation of xylem water from sapwood cores but not cryogenic vacuum extraction. The datasets are water stable isotope measurements of precipitation, xylem, groundwater, and vadose zone pore water samples from the site. Each data file is formatted as a CSV and includes time-stamped observations. Below, we describe a list of column names that applies to each of the isotope datasets. Water Stable Isotope Data Filenames: Groundwater_isotopes.csv Groundwater from wells Solid_isotopes.csv Soils and weathered bedrock dry augered Precip_isotopes.csv Precipitation VMS_isotopes.csv: Vadose zone pore waters from the Vadose Zone Monitoring System Tree_isotopes.csv: Tree samples Description: These files contain water stable isotope measurements, along with sample metadata, collection details, and site information. Columns: Sample Information: ERCZO ID: Unique identifier for the sample. Original Sample Vial Label: Label assigned to the sample vial. Date Collected: Date the sample was collected (YYYY-MM-DD). Time Collected: Time the sample was collected (HH:MM). Site: General site location of the sample. Location: Specific sampling location within the site. Sample Type: Type of sample collected (e.g., groundwater, xylem water). Sample Type II: Additional classification of the sample. Tree Tag, Tree tag, Tree tag 2: Identifiers for sampled trees (if applicable). Environmental & Collection Metadata: Species (common), Species (scientific): Common and scientific names of sampled vegetation. Well water level (m): Groundwater level in the well at the time of sampling (m). Depth (m), Depth (cm), Depth (ft): Sample collection depth in different units. Gravimetric moisture content (g/g): Gravimetric moisture content measurement. Collected by: Name of the collector. Sampling Notes: Additional notes on the sampling process. Isotope Analysis & Instrumentation: dD Instrument, dO Instrument: Instruments used for ?D and ???O measurements. HD run ID, GB run ID: Run IDs for isotope analysis. dD: Measured ?D value. dO: Measured ???O value. Additional Metadata: Sample Vial Location: Storage location of the sample. VMS Sleeve, VMS Port: Identification for VMS sampling ports. Adjacent well groundwater depth at time of sampling (m): Nearby well water level during collection. Energetic State: Energy state classification of the sample. Date collection started, Time collection started: Timestamp of sample collection initiation. Extraction Method: Method used for extracting water. Centrifuge Duration (hr), Water Centrifuge Duration (hr): Time spent centrifuging the sample. Analysis Notes: Additional comments related to analysis. timestamp, timestamp_rounded: Exact timestamps of data recording. waterYear: Water year corresponding to the observation. date, datetime: Alternative date formats for ease of analysis. Usage Notes All timestamps are in YYYY-MM-DD format unless otherwise specified. Missing values are represented as NaN. Please cite the dataset properly when using it in research publications.
Credits
Funding Agencies
This resource was created using funding from the following sources:
Agency Name | Award Title | Award Number |
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National Science Foundation | Tracing the fate, age, and ecohydrologic significance of rock moisture | 2100760 |
How to Cite
This resource is shared under the Creative Commons Attribution CC BY.
http://creativecommons.org/licenses/by/4.0/
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