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| Type: | Resource | |
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| Created: | Aug 06, 2025 at 2:54 a.m. (UTC) | |
| Last updated: | Apr 04, 2026 at 1:34 a.m. (UTC) (Metadata update) | |
| Published date: | Apr 04, 2026 at 1:34 a.m. (UTC) | |
| DOI: | 10.4211/hs.3b2886a7bade49dabc5a7d1413b73681 | |
| Citation: | See how to cite this resource |
| Sharing Status: | Published |
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Abstract
This study was conducted in the Paint Rock watershed (outlet: 34.9686, -86.1650) which lies on private land in Jackson County, AL, near Estillfork. It drains a non-perennial tributary to Burks Creek and spans 2.97 km² of deciduous forest in the Cumberland Plateau. Elevation ranges from 211 to 550 m, and the watershed feeds into the Paint Rock River (Tennessee basin). The climate is humid subtropical, with mean January and July temperatures of 4.4°C and 25.4°C, and annual precipitation of 1,390 mm. This watershed was included in a seasonal sampling survey (AIMS Approach 2) conducted from January 2022 to January 2024 as part of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. The field team collected data on hydrology, biogeochemistry, and microbial ecology at 7 sites within each watershed. Site selection balanced three priorities: (1) targeting locations with existing long-term monitoring infrastructure; (2) including sites near springs and tributary confluences; and (3) capturing variability in drainage area and topographic wetness index (TWI), which are linked to flow permanence. At each site, samples of water (when present), biofilm, leaf litter, and sediment were collected. Microbial enzyme activities were measured to assess organic matter decomposition (β-glucosidase, phenol oxidase, peroxidase) and nutrient mineralization (N-acetylglucosaminidase for nitrogen, phosphatase for phosphorus). Enzyme activities were quantified using fluorometric methods with 4-methylumbelliferyl (MUB) substrates or colorimetric methods using 4-nitrophenyl (pNP) and L-DOPA substrates.
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Coverage
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Credits
Funding Agencies
This resource was created using funding from the following sources:
| Agency Name | Award Title | Award Number |
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| U.S. National Science Foundation | Aquatic Intermittency Effects of Microbiomes on Streams | 2019603 |
How to Cite
This resource is shared under the Creative Commons Attribution CC BY.
http://creativecommons.org/licenses/by/4.0/
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