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| Created: | Jun 25, 2024 at 6:54 p.m. | |
| Last updated: | Jul 26, 2024 at 6:22 p.m. | |
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Abstract
The following standard operating procedure (SOP) was created for the the Aquatic Intermittency effects on Microbiomes in Streams (AIMS), an NSF EPSCoR funded project (OIA 2019603) seeking to explore the impacts of stream drying on downstream water quality across Kansas, Oklahoma, Alabama, Idaho, and Mississippi. AIMS integrates datasets on hydrology, microbiomes, macroinvertebrates, and biogeochemistry in three regions (Mountain West, Great Plains, and Southeast Forests) to test the overarching hypothesis that physical drivers (e.g., climate, hydrology) interact with biological drivers (e.g., microbes, biogeochemistry) to control water quality in intermittent streams. An overview of the AIMS project can be found here: https://youtu.be/HDKIBNEnwdM
This protocol will detail the process for collecting and storing dissolved gas samples to be analyzed on the Membrane Inlet Mass Spectrometer (MIMS) for the biogeochemistry theme. MIMS samples will be collected during seasonal sampling (Approach 2) and synoptic sampling (Approach 3). From this SOP, the following data types will be created: Concentrations of Nitrogen (N2), Oxygen (O2), and Argon (Ar) in μM. [AIMS rTypes: MIMS, GASS]
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