This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 7 October 2021 and 30 January 2023, grab samples for Total Suspended Solids (TSS, mg/L) and seston Ash Free Dry Mass (AFDM, mg/L) were collected at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3). Additional "stretch" samples were collected for TSS and AFDM at a tributary (TLC01) and downstream perennial monitoring site (TLP01), which are included in a separate file from the core TSS data from the Talladega research watershed. To measure TSS and AFDM concentrations, we collected 2- 3 replicates of unfiltered stream water in clean 1L Nalgene bottles after triple rinsing in the field, stored on ice, and brought to the lab for filtering. We brought samples back to the lab and filtered samples in 100mL batches through pre-ashed (550℃ for one hour) and pre-weighed 47 mm Whatman GF/F filters. Once the sample was filtered, the filters were placed in a drying oven for at least 48 hours and weighed. TSS was calculated as the (dry weight (filter + tin + sample) - dry weight (filter + tin)) / volume filtered (L). Ash free dry mass (AFDM) was then calculated as (dry weight (filter + tin + sample) - ashed weight (filter + tin + sample)) / volume filtered (L).