This study characterizes seasonal hydrological, biogeochemical, and microbial dynamics across three watersheds in Alabama as part of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project (Approach 2 seasonal sampling survey, January 2022-January 2024). The Weyerhaeuser watershed (outlet:32.9841, -88.0133; Greene County) drains a non-perennial tributary to Shambley Creek across 0.7 km² of silviculturally managed coniferous forest in the East Gulf Coastal Plain (elevation 63-94 m), ultimately contributing to the Sipsey River within the Mobile-Tombigbee basin. The Paint Rock watershed (outlet: 34.9686, -86.1650; Jackson County) drains a non-perennial tributary to Burks Creek across 2.97 km² of deciduous forest in the Appalachian plateau (elevation 211-550 m), draining into the Paint Rock River within the Tennessee River basin. The Talladega watershed (outlet:33.7622, -85.5955; Cleburne County) drains a non-perennial tributary of Pendergrass Creek across 0.92 km² of mixed coniferous-deciduous forest in the Piedmont region (elevation 345-456 m), draining into the Coosa River within the Mobile-Tombigbee basin. All three sites share a humid subtropical climate, with mean January/July temperatures and annual precipitation ranging from 4.4-7.3 °C, 25.3-27.4 °C, and 1350-1400 mm, respectively. At each watershed, seven sites were sampled to balance existing long-term monitoring infrastructure, proximity to springs and tributary confluences, and variability in drainage area and topographic wetness index - metrics linked to flow permanence. Sediment samples were collected and sediment nutrient (ammonium, nitrate and orthophosphate) concentrations were quantified by extracting 10g of sediment with degassed deionized water, followed by centrifugation and filtration; extracts were analyzed through colorimetric microplate assays (indophenol blue for ammonium, molybdenum blue for orthophosphate, and vanadium chloride/Griess method for nitrate), with concentrations calculated from standard curves and reported per gram of dry sediment. Sediment extract pH was also measured.